I.3 The polarising microscope

 

Parts of the microscope:

 

 

 

 

 

Objectives: these are the lenses used to magnify the specimen on the stage. Do not let the objectives ever touch your slides. To change magnification, rotate the nosepiece on which the objectives are fitted. Touching the objectives will put the centering out and requires repair by a specialist… so don’t touch them!

 

Rotating stage: the stage can be rotated 360° and is graduated in degrees. For most microscopes the stage diameter is 120 mm.

 

Condenser: the polarising condenser is in a cylindrical sliding mount with swing-out top part and aperture iris diaphragm. Normally the condensing lens is swung out, and only swung in, when viewing an interference figure. The condenser varies the amount of light hitting the specimen.

 

Polariser: vertically removable filter polariser in rotatable mount. Readings are at intervals of 90°. Note that the polariser is below the stage, and allows light to pass through it vibrating E-W. The polariser is normally left in this position.

 

Focussing: the image is focused by raising or lowering the rotating stage. There are single-knob coarse and fine focussing controls on both sides of the stand.

 

Interchangeable tube: the eyepiece ocular is the lens fitted to the top of this tube. The eyepiece magnifies and focuses the image produced by the objective lens. The eyepiece assembly contains two cross-hairs which should be oriented E-W and N-S (parallel to the vibration directions of the polariser and the analyser). The cross-hairs can be focussed by raising or lowering the eyepiece in its mount. The eyepiece have a standard magnification of 10x or 8x. The magnification produced by both eyepiece and objective is obtained by multiplying the two separate magnifications.

 

The Bertrand lens: the Bertrand lens magnifies and focuses interference figures. It swings in and out.

 

Pinhole stop: swings in and out, for singling out small object details in conoscopic observations.

 

Analyser: Removable filter polariser that can swing in and out. It allows light to pass through vibrating N-S, i.e. perpendicular to the polariser vibration direction. Periodically check that the polariser and analyser vibration directions are at right angles to each other.

 

Revolving nosepiece: use this nosepiece when changing to a higher or lower magnification. Pushing the objectives can cause damage to the objectives and will put the centering out.

 

Accessory plates: accessory plates are used for studying interference figures and the retardation produced by mineral specimens. When required, they are inserted into the microscope tube in a slot between the objectives and the analyser. One standard accessory plate with the microscope is the gypsum plate.

 

When one leaves out the polarisers, the orthoscopic image formation is the same as in a normal microscope. (Fig. 1.4). Light from a (low voltage) microscopy lamp is comprimated through the collector lens, which is build in the lamp holder. The light is polarised by the polariser. Then it passes through the aperture diaphragm and the condenser, which determines the intensity and the top angle of the through the sample going lightcone. The desired top angle depends basically on the aperture of the chosen objective.  Now the light passes through the sample, objective and analyser, respectively. The vibration direction of the analyser is perpendicular to that of the polariser. Finally it passes through the eyepiece. This is called orthoscopic because the light rays are roughly parallel and vertical as they pass through the sample, which  is the determining factor for the image formation.

 

This is different when using conoscopic viewing, which is obtained by bringing in the condensing lens below the stage and the Bertrand lens above it. Now the eye does not see points of the object but directions through the object. The centre of the image now represents the properties of light going through the sample perpendicularly, while the edges of the image represent the outer radiation of the through the object going light cone. For further explanation see chapter IV.

 

I.3.1 Orthoscopic and conoscopic viewing

 

The petrographic or polarising microscope is the most important instrument for the determination of the optical properties of minerals and their textural relationships. Microscopy is the easiest, quickest and least expensive way of obtaining information about minerals. The image formation in a normal microscope is considered to be known.